| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252964 |
| Chromosome: | chromosome 9 |
| Location: | 7115646 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411850 | (1 of 2) PTHR23130:SF61 - DEFENSE PROTEIN L(2)34FC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCTCTTCGTATACCGTATGACCTGGT |
| Internal bar code: | CTCTGACAGACATTCGGCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 99.5329 |
| LEAP-Seq n confirming: | 2131 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAAACGCTTGTTTGGGC |
| Suggested primer 2: | CGTGTGGCTTGGTATGTTTG |