| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252967 |
| Chromosome: | chromosome 1 |
| Location: | 6073307 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043250 | (1 of 3) PTHR30570:SF0 - PHOSPHATE-BINDING PROTEIN PSTS | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAGTCTGCGCAGTGCCGCCATCAGAGT |
| Internal bar code: | TCAGGAGGAAATGTGCTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 663 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCATCACCGTGTTCTAC |
| Suggested primer 2: | ACAGACAACCAAGGGTGGAG |