| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252969 |
| Chromosome: | chromosome 12 |
| Location: | 329956 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494600 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCACAACAATGCGGCCCTCAACGCTCA |
| Internal bar code: | TTCCCACGTCATAACCTAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 99.7732 |
| LEAP-Seq n confirming: | 3079 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGACTGCCCTTTCTTCG |
| Suggested primer 2: | GCAGGAACTGTCATTGGGTT |