Insertion junction: LMJ.RY0402.252973_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252973
Chromosome:chromosome 17
Location:6845169
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g745347 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGTATCATATCGGTACCATATCGGACT
Internal bar code:GGTACCTCTCAAAAGCCGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:486
LEAP-Seq percent confirming:99.1939
LEAP-Seq n confirming:2215
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACATCATCGTGTTCTGC
Suggested primer 2:CCTTATCCTACAGCCCTCCC