| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253028 |
| Chromosome: | chromosome 8 |
| Location: | 3949897 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378601 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCGGCCTGTGGTCTTCACGCGTTGCC |
| Internal bar code: | CGTCCAGTCTTTTGTTAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 99.7286 |
| LEAP-Seq n confirming: | 735 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGCACACATTCTTAAA |
| Suggested primer 2: | CCAGCACCTAGCTGGATACC |