Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.253046 |
Chromosome: | chromosome_3 |
Location: | 5012699 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g180550 | NTD5,FPN2 | 5\'-nucleotidase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGATGGGGAAGGAAGGAGGGGTGCTTGAAG |
Internal bar code: | CGGCACGCACGCCTTCGGACCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 496 |
LEAP-Seq percent confirming: | 97.6744 |
LEAP-Seq n confirming: | 84 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACACGTAGTGGTGGTCGTG |
Suggested primer 2: | CCACCGTTGTCACATACCAG |