| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253068 |
| Chromosome: | chromosome 6 |
| Location: | 2606443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269601 | (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGATTGTAATCATTCATATGGTACCGTCA |
| Internal bar code: | CAAAGGTACAACCGAACGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1143 |
| LEAP-Seq percent confirming: | 99.5281 |
| LEAP-Seq n confirming: | 2531 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGCCGACTGCTTTGGTG |
| Suggested primer 2: | GGAGCAAATTGCGCTCTAAC |