| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.253069 |
| Chromosome: | chromosome 17 |
| Location: | 187643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697500 | P25 Alpha Domain and EF Hand Protein; (1 of 2) PTHR12932:SF9 - TPPP FAMILY PROTEIN CG45057 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGAAGTTGCTCGCGAGTCGAGTCGGGT |
| Internal bar code: | GTCCGCGTCGTGCGGGCTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 96.0422 |
| LEAP-Seq n confirming: | 364 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTCCAAGGAAAGGGAG |
| Suggested primer 2: | GCTGAGCTTCAGGAAGGAGA |