Insertion junction: LMJ.RY0402.253069_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.253069
Chromosome:chromosome 17
Location:187650
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697500 P25 Alpha Domain and EF Hand Protein; (1 of 2) PTHR12932:SF9 - TPPP FAMILY PROTEIN CG45057 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGGCGCGTACCGGTGAGCTTACCACAT
Internal bar code:AAACGTATTAGCCATCGTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:807
LEAP-Seq percent confirming:98.2206
LEAP-Seq n confirming:5520
LEAP-Seq n nonconfirming:100
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGTCCAAGGAAAGGGAG
Suggested primer 2:GCTGAGCTTCAGGAAGGAGA