| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253072 |
| Chromosome: | chromosome 13 |
| Location: | 1200555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569950 | HTR2 | Histone H3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGATATTTGCGGATCTCACGCAGCGCC |
| Internal bar code: | GTTTCTTTCCCCCTGACTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 99.5763 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACAAAGAGCAGAAGTCC |
| Suggested primer 2: | AGCAAGGGACTAAAGTGCGA |