Insertion junction: LMJ.RY0402.253073_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.253073
Chromosome:chromosome 3
Location:333905
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g144707 (1 of 1) 5.4.2.2//5.4.2.8 - Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) / Phosphoglucose mutase // Phosphomannomutase / Phosphomannose mutase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGGAATACTCCCTCGCAGCCTCCTCGT
Internal bar code:TGAGGGAACAGCGCGCATCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:50
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:191
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTGTGACCGTGACTTGG
Suggested primer 2:ATGGTGGTGAGAAGGGTGAG