| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.253096 |
| Chromosome: | chromosome 1 |
| Location: | 2949010 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018000 | RPA12 | DNA-directed RNA polymerase I subunit A12.2; (1 of 1) K03000 - DNA-directed RNA polymerase I subunit RPA12 (RPA12, ZNRD1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGTCACAACCGTCAGCATGGTACTACG |
| Internal bar code: | ATCCAAGCAAACAACCCTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 99.0164 |
| LEAP-Seq n confirming: | 302 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGACCGTCTTCTACGAG |
| Suggested primer 2: | GTGGAGAATCGGTCACAGGT |