| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253102 |
| Chromosome: | chromosome 1 |
| Location: | 5260194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036700 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGGCTGAACGCCTGGGCGTGGACGCAT |
| Internal bar code: | AGCCTTAGTTCAGGTGACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1172 |
| LEAP-Seq percent confirming: | 99.7248 |
| LEAP-Seq n confirming: | 2174 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAGAGCCCTGCATTCTGC |
| Suggested primer 2: | AGCCCGGTTCAACCTTCTAT |