| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253110 |
| Chromosome: | chromosome 10 |
| Location: | 4071959 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449501 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATAATTGCCCCAGTGGGCTTACGCCGGA |
| Internal bar code: | CGGGGGCTACAACCCCTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 9.90026 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 4878 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCATGACATGTTCGCAT |
| Suggested primer 2: | AAGCCACTAGGGCATCATTG |