| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253138 |
| Chromosome: | chromosome 12 |
| Location: | 5743266 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533000 | PHR2,PHR1 | (1 of 3) K01669 - deoxyribodipyrimidine photo-lyase [EC:4.1.99.3] (phrB); CPD photolyase, class II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCCACTACTGCCCCACATACGACAGCC |
| Internal bar code: | CCAATATACGAGGGGTACGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 79.4118 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATGTGGATGTTGGAGG |
| Suggested primer 2: | CTATACACAACCGCTTCCCC |