| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253145 |
| Chromosome: | chromosome 12 |
| Location: | 3282765 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498100 | EIF3E | (1 of 1) K03250 - translation initiation factor 3 subunit E (EIF3E, INT6); Eukaryotic translation initiation factor 3, subunit E | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTGGCACAGCGTTCACACCTACATCCA |
| Internal bar code: | GAGTTTGATGCCATGGCCTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 99.3357 |
| LEAP-Seq n confirming: | 5682 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGGTGCTGATGAGATGA |
| Suggested primer 2: | TGCAGGATGGATAGGGGTAG |