Insertion junction: LMJ.RY0402.253189_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.253189
Chromosome:chromosome 7
Location:6397549
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g357900 (1 of 1) K08054 - calnexin (CANX) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCACTCCTCAGGCTTCTTGGCGGCTGA
Internal bar code:TACCACTTTGTAAATGAGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:153
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTTGCGGTTCTTTTTGC
Suggested primer 2:GAAAGTTCACACGGGTCCAT