| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253432 |
| Chromosome: | chromosome 10 |
| Location: | 3063740 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441500 | (1 of 1) K09602 - ubiquitin thioesterase protein OTUB1 [EC:3.4.19.12] (OTUB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGGGTGCTGCCGCTGTCACCCGCTCT |
| Internal bar code: | CGTACGATTTTGTGGTTCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.6347 |
| LEAP-Seq n confirming: | 3546 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCTGAGGAAAGCACAA |
| Suggested primer 2: | TGTGCAGCTTTGATGAGACC |