| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.253446 |
| Chromosome: | chromosome 16 |
| Location: | 2921513 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663950 | ERG3 | Delta-7-sterol-C5(6)-desaturase; (1 of 1) K00227 - lathosterol oxidase (SC5DL, ERG3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCTGCTACTTCCCGGCAGGTACCTGCA |
| Internal bar code: | ATTCTTACTGGAGAGGGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.1803 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGCGGGTAGGTGTCAAG |
| Suggested primer 2: | TCGTGGATGTTGGTAGTCCA |