Insertion junction: LMJ.RY0402.253450_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.253450
Chromosome:chromosome_3
Location:8043677
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGGGAGTCTGGGACGCGATGGGCATGAGGG
Internal bar code:TTCCTTCGCATGTCCGTATTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:50
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACGAGAAGTAAAAGGGGGC
Suggested primer 2:GAGGTGGTGCGGTTCTGTAT