| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.253481 |
| Chromosome: | chromosome 12 |
| Location: | 2529656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505900 | TIM50 | (1 of 1) K17496 - mitochondrial import inner membrane translocase subunit TIM50 (TIM50) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCGTGCATGACGGGAAAGGGCTGTAC |
| Internal bar code: | TCGGAGTTACGTGTCCTGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 98.495 |
| LEAP-Seq n confirming: | 1767 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCCAAGGGTTAGTCTGT |
| Suggested primer 2: | TCAGTGAAGTCCTTGATGCG |