Insertion junction: LMJ.RY0402.253487_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253487
Chromosome:chromosome 3
Location:4303644
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174750 CYN26,CYN26B,CYN26-2 (1 of 1) PTHR11071//PTHR11071:SF244 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 26-2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTACACGCCGGTCCGTGTGCCCCGTGCG
Internal bar code:ACGTTGGCGTGGAGGGAAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:607
LEAP-Seq percent confirming:99.688
LEAP-Seq n confirming:639
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCATCCCTGATTCCTCA
Suggested primer 2:GGATACGGTTTGGCGATAGA