Insertion junction: LMJ.RY0402.253489_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.253489
Chromosome:chromosome_13
Location:4607093
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTTTCGTCTTTGGCGTTTTGTGTCTCTCGG
Internal bar code:CGGTCACAGGCATGGACAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:219
LEAP-Seq percent confirming:98.9053
LEAP-Seq n confirming:6776
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCGCGTACAGGTATGGAC
Suggested primer 2:GTTAGCTCCCCTCTCCAACC