| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253499 |
| Chromosome: | chromosome 17 |
| Location: | 18098 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696250 | (1 of 2) K03260 - translation initiation factor 4G (EIF4G) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTAGCTAGTTAGGTAGCATTGCATTAA |
| Internal bar code: | GGTAAATCCCATGCGGATATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 99.8353 |
| LEAP-Seq n confirming: | 606 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAAGTCGGCTTTGTTTCT |
| Suggested primer 2: | AAGGTTTTGCACCCAACAAG |