| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253511 |
| Chromosome: | chromosome 1 |
| Location: | 1708334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009101 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTTGGGCCGGTGCCTCATGTTGCCGT |
| Internal bar code: | ACCTAGGTTGCACGTTTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 98.6261 |
| LEAP-Seq n confirming: | 2369 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCTGTGCGAGTGAAAA |
| Suggested primer 2: | CGGCTCTTGGTGTAAATGGT |