Insertion junction: LMJ.RY0402.253581_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253581
Chromosome:chromosome 5
Location:1400318
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g244250 DHC6 Axonemal Dynein Heavy Chain 6; (1 of 3) PTHR10676//PTHR10676:SF242 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 3, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGAACACAAACATGCCGTCGAGCTGCTT
Internal bar code:GCGGATTCGGTTGACCCGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:449
LEAP-Seq percent confirming:99.3174
LEAP-Seq n confirming:291
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCACAAACACCACACAGC
Suggested primer 2:TGACAACAAGAAGCTGTGCC