| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253598 |
| Chromosome: | chromosome 2 |
| Location: | 5744658 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110400 | (1 of 1) PTHR12732:SF0 - PCI DOMAIN-CONTAINING PROTEIN 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCTAGTCTATGGCCAAGCTCTTCTAGCT |
| Internal bar code: | ATATACTCTGTTCTGACGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 99.2453 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGCTGACCGGTTTTATG |
| Suggested primer 2: | GTCACCGTCACACACAGGTC |