| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253606 |
| Chromosome: | chromosome 16 |
| Location: | 1565992 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCGCAAGGCCAAGTGAGTGACCTCAGG |
| Internal bar code: | TTAGACAACGCGTACAGATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 99.2064 |
| LEAP-Seq n confirming: | 875 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTGTTCCTGAGCACTCC |
| Suggested primer 2: | TTTTCTTAAAGGTGCGGGTG |