| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253632 |
| Chromosome: | chromosome_10 |
| Location: | 4406709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g451500 | SRR28,CHI5 | Chitinase-like hydrolase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCCCCATAGTAGCCGGTGCGGCTGCCGC |
| Internal bar code: | TAATGCTATGATCGGTCGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 92.6335 |
| LEAP-Seq n confirming: | 503 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGACCACCACATCCAG |
| Suggested primer 2: | CGCTCATTGTCTCCAAGTCA |