| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253646 |
| Chromosome: | chromosome 3 |
| Location: | 1580320 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g152500 | (1 of 5) IPR000104//IPR001214 - Antifreeze protein, type I // SET domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTTGGGTGAGAGCGCAACCCTCCAAGA |
| Internal bar code: | ACGTCACTTCAGGTTTTGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCAAGACTGGCGGTAG |
| Suggested primer 2: | ACTCCACCGTATTCAGGCAC |