| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253692 |
| Chromosome: | chromosome 8 |
| Location: | 4234784 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380600 | (1 of 1) K08287 - dual-specificity kinase (E2.7.12.1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGTTTTGGGGGGTGGGGGTGGAGGTGG |
| Internal bar code: | AATAGGGGAGTGAACTTCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 86.0119 |
| LEAP-Seq n confirming: | 289 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACAAAGCGGAACTAGCG |
| Suggested primer 2: | ACCCCACACCTCCTCCTC |