Insertion junction: LMJ.RY0402.253715_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253715
Chromosome:chromosome 14
Location:688899
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g612500 (1 of 1) K05954 - protein farnesyltransferase subunit beta (FNTB) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGAATCAGGTTTGGCGTGGAGCGTGCT
Internal bar code:GAGGGGGATTCCAGAACTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:496
LEAP-Seq percent confirming:96.9283
LEAP-Seq n confirming:3976
LEAP-Seq n nonconfirming:126
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTCAACGTGGTGGATGAC
Suggested primer 2:ATTAGTTGCAATCGGCCAAC