| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253771 |
| Chromosome: | chromosome 1 |
| Location: | 1857457 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010150 | (1 of 3) IPR006073//IPR027417 - GTP binding domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGATTCAATTGCGCTGGTACAATATGA |
| Internal bar code: | ACCCGGTCTAGGTAAGTCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTTGGCAAGGAACTTGG |
| Suggested primer 2: | GCACATGAAGACATGCTGCT |