| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253777 |
| Chromosome: | chromosome 17 |
| Location: | 2381778 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715100 | (1 of 1) PF15249 - Conserved region of unknown function on GLTSCR protein (GLTSCR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGTGTTCAAGCAGCTAGTAGCGACCCC |
| Internal bar code: | CCTGCGTCGGTGTCTTACAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 99.6728 |
| LEAP-Seq n confirming: | 4570 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGAACATGTGACGGTGA |
| Suggested primer 2: | ACGGAAAACAGCAAAACCAC |