| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253782 |
| Chromosome: | chromosome_17 |
| Location: | 3954981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728650 | FAP196 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTAGGCAAAAAAACCGGTTCTCACTACCCC |
| Internal bar code: | CACAACATAGTGACGCGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 76.0512 |
| LEAP-Seq n confirming: | 416 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTCGCTGATACACGCT |
| Suggested primer 2: | ACACACACACACACACACCG |