Insertion junction: LMJ.RY0402.253797_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253797
Chromosome:chromosome 17
Location:1147009
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g704300 FAP47 Flagellar Associated Protein 47; (1 of 1) PTHR23053//PTHR23053:SF2 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // PRIMARY CILIARY DYSKINESIA PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCTTGATGGCGGGTGCGTGTTTAAGGTC
Internal bar code:AAAGGACGGCGGTTGCGAACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:472
LEAP-Seq percent confirming:83.2504
LEAP-Seq n confirming:502
LEAP-Seq n nonconfirming:101
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTAACACGCTTCTCTTGCCC
Suggested primer 2:CTTAGGTGGTTGCAGGAAGC