| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253803 |
| Chromosome: | chromosome 10 |
| Location: | 4789066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453800 | TSP3,CTPA3,TCP3 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2; putative Sphingomyelin phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCACCGGGAAGGGGGGGGGGGACACGGT |
| Internal bar code: | TTTTGCTGTCTGCGCACGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAGCTAGGCACCCTATT |
| Suggested primer 2: | CAATCCAGCAACAAGGGTTT |