Insertion junction: LMJ.RY0402.253813_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253813
Chromosome:chromosome 16
Location:123093
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCAACTTCCGCGTCACTTGGACATCAT
Internal bar code:CGAAGGGGGTTTGCGCCGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:566
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTGAGGGGTTGGTTACA
Suggested primer 2:TTGTGGAAGCACTCTTGACG