Insertion junction: LMJ.RY0402.253822_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.253822
Chromosome:chromosome 11
Location:977480
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467664 (1 of 3) K17285 - selenium-binding protein 1 (SELENBP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCATGCTCTCGTCCGCGCTCAGCCGCAC
Internal bar code:AATCCCGATTATCATTTTTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:528
LEAP-Seq percent confirming:98.9796
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCGCTGAGCACAAGAAT
Suggested primer 2:AATCCTTAAACCATGCACGC