| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253830 |
| Chromosome: | chromosome 1 |
| Location: | 4119252 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027100 | IMP2 | Mitochondrial inner membrane signal peptidase; (1 of 1) K09648 - mitochondrial inner membrane protease subunit 2 (IMP2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCTGTGGATGTGCGGGGCTTCCGCTGG |
| Internal bar code: | GCCCGTCCTAAATTCCGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGCATCGAGCAGGTCC |
| Suggested primer 2: | TGCCGGTGTAGCTTGATATG |