Insertion junction: LMJ.RY0402.253837_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253837
Chromosome:chromosome 3
Location:9068683
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g210737 (1 of 726) IPR011009 - Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCATCTCGCACAAACCGTGAAACCGCT
Internal bar code:CTAGACTACGAGGCATCGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:540
LEAP-Seq percent confirming:88.9098
LEAP-Seq n confirming:473
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATCAACGAACTTGCTGC
Suggested primer 2:GCTCCTCCGTGTACTCCGT