| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.253838 |
| Chromosome: | chromosome 10 |
| Location: | 4027161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449200 | ANK5 | (1 of 1) PTHR22849//PTHR24133//PTHR24133:SF23 - WDSAM1 PROTEIN // FAMILY NOT NAMED // SUBFAMILY NOT NAMED; Predicted protein with ankyrin repeats | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCAGGTGGCCGAGCGTGGAGGCTTTGG |
| Internal bar code: | GCTTCTACCTTGGTGTGATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 98.3425 |
| LEAP-Seq n confirming: | 356 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATCGTTTGCATTGAGG |
| Suggested primer 2: | GCTGCTATCATGGATTGGCT |