| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253850 |
| Chromosome: | chromosome 3 |
| Location: | 5153562 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182150 | TEF8 | (1 of 1) PTHR30373:SF2 - UPF0603 PROTEIN YGCG; Thylakoid Lumen Protein 18.3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCAGGTGCTGGAGAACTGGTACCCCAC |
| Internal bar code: | CTGAATCAGCTTAGAGAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 96.1712 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAACCACAGAAACCATGC |
| Suggested primer 2: | GGCTATCGTAGCTACTGGCG |