Insertion junction: LMJ.RY0402.253871_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.253871
Chromosome:chromosome 17
Location:4096176
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729400 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGACCACACAACCACAGGCGAAACAT
Internal bar code:CCTGTCGCGTTAGCTTTACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:115
LEAP-Seq percent confirming:40.9091
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGGAAGGAGTTGTTGGG
Suggested primer 2:GAGAGGAAGCTTGAGACGGA