| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.253875 |
| Chromosome: | chromosome 17 |
| Location: | 1697429 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708600 | HTB27,HTB20 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACACACAGCTCCTGAGCTCAGTGGCC |
| Internal bar code: | TCGGGGATGCTGGAGTGTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 96.8519 |
| LEAP-Seq n confirming: | 523 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCAAACAAACTCGGTGT |
| Suggested primer 2: | GGACGCCTGTGCTAAGTCTC |