| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.253879 |
| Chromosome: | chromosome 11 |
| Location: | 2832532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCCGAATGTGAATGCGCAATGAGGCT |
| Internal bar code: | TCCTGGTTTCGACAAGTGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 99.6141 |
| LEAP-Seq n confirming: | 6454 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACAGCGAGATGGACGA |
| Suggested primer 2: | TTCTGATGGTCGATTCTCCC |