| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.253910 |
| Chromosome: | chromosome 10 |
| Location: | 5523603 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459350 | OPR49,RAP5 | OctotricoPeptide Repeat protein 49; (1 of 2) IPR000104//IPR013584 - Antifreeze protein, type I // RAP domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGAGGGGGGGGGCTCACCCTGCCGCGTA |
| Internal bar code: | GGACAGGACGCGGGTGAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 98.1037 |
| LEAP-Seq n confirming: | 776 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACAACGCACAACAATCC |
| Suggested primer 2: | GGTTGCAAGGTCAGAAGAGC |