Insertion junction: LMJ.RY0402.253949_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.253949
Chromosome:chromosome 12
Location:8799056
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g545250 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGCGCCTCTCCTCCCGCTCCGCACGCT
Internal bar code:ATGTGGGCCGTGAGCCGAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:67
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACTAGTGGAATGGGCTC
Suggested primer 2:GACACCCCACTACACATCCC