| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253989 |
| Chromosome: | chromosome_16 |
| Location: | 1509485 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g653100 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAATTCCATTTGTGCAGACGCTCTGCGC |
| Internal bar code: | AAGGAGCGCACTCGGAAGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.115 |
| LEAP-Seq n confirming: | 224 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAGCAGTAGCAGTGAC |
| Suggested primer 2: | TCGCGGCTATAAGATCCAAC |