Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.254001 |
Chromosome: | chromosome_5 |
Location: | 1057193 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g246200 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCCCAGCTCGGAAGCGCGCTTCCGCTCCG |
Internal bar code: | GCCGACTTTTAATTGTCCCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 159 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 64 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTCCATCCTCCTCGTCTG |
Suggested primer 2: | AGGACCCTTCACATCTGCAC |